Background: Most women in reproductive age group have one or more emotional and physical symptoms in the premenstrual phase of the menstrual cycle, which are grouped as premenstrual tension (PMT) or premenstrual syndrome (PMS). Alterations in the concentration of various minerals have been associated with many affective disorders. Recently it has been proposed that the fluctuation in serum calcium and serum magnesium level in the luteal phase of menstrual cycle might be responsible for multiple symptoms in women with premenstrual syndrome. Aim:: Theaim of the study was to assess the serum calcium and serum magnesium level during the luteal phase of menstrual cycle in women with premenstrual syndrome. Materials and Methods: It was a cross sectional study conducted among 70 unmarriedwomen of 18-25 years after obtaining ethical committee clearance. Questionnaire was explained to the participants in their native languageafter obtaining informed written consent. Premenstrual Syndrome Scale (PMSS) was structured in such a way to obtain information about various symptoms of Premenstrual Syndrome. About 3 ml of venous blood was collected duringluteal phase of menstrual cycle for serum calcium and magnesium level estimation. The data thus obtained were tabulated and analyzed statistically by using SPSS software. Results: Serum calcium and magnesium level were reduced in the PMS group significantly (p value - <0.05) but the calcium level was within the normal range. Of all study population 5.7 % of women had hypocalcemia and 34.3% had hypomagnesemia. Conclusion: There is a statistically significant fall incalcium and magnesium levels in women with premenstrual syndrome.